ABSTRACT
Objective To establish an intelligent and integrated regional health service platform by process reengineering, multi business system collaboration,heterogeneous data exchange and applying big data analysis.Methods Interconnection, intercommunication and data sharing between related platforms were implemented with service-oriented architecture,internet technology, integrated medical resources in Nanjing as well as combined on-and off-line services. Results The regional health service platform based on big data sharing was implemented in Nanjing so that medical service process was optimized and the problems such as"three longs and one short"were solved.The idea and methods for constructing the platform were recognized by national 12320 center and duplicated by six provinces, and social benefits were gained. Conclusion It's feasible to establish the regional health service platform based on big data sharing, and whole-course and multi-way self health management is implemented.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of transfecting DHFR (human double-mutant dihydrofolate reductase) gene into mouse bone marrow cells and the effect of resistance to high dose MTX chemotherapy.</p><p><b>METHODS</b>After DHFR gene was transfected into mouse bone marrow cells with retroviral vector, the cells were treated with methotrexate (MTX) and then CFU-GM (granulocyte-macrophage colony-forming unit) assay was performed. Peripheral blood leucocytes and platelets, body weight and survival rate were observed. After treatment with high dose MTX, the expression of drug resistance gene was checked by RT-PCR in the transfected bone marrow cells.</p><p><b>RESULTS</b>SFG-F/S-NeoR gene-transfected mice bone marrow cells yielded drug-resistance colonies to MTX (donor mice: 15.8%, recipient mice: 18.0%, control: 0) The peripheral blood leucocytes and platelets, body weight recovered gradually and the survival rate was 83.3% at the 40th day, while 0 in controls in gene transfected mice after large dose MTX treatment. RT-PCR of transgenic mouse marrow cells showed the band of F/S gene (400 bp).</p><p><b>CONCLUSION</b>DHFR gene can not only be integrated and expressed in bone marrow cells but also improve their drug-resistence to MTX.</p>
Subject(s)
Animals , Male , Mice , Antimetabolites, Antineoplastic , Pharmacology , Bone Marrow Cells , Cell Biology , Metabolism , Bone Marrow Transplantation , Cells, Cultured , Drug Resistance, Neoplasm , Genetics , Erythrocyte Count , Genetic Vectors , Leukocyte Count , Methotrexate , Pharmacology , Mice, Inbred BALB C , Mutation , Retroviridae , Genetics , Survival Analysis , Tetrahydrofolate Dehydrogenase , Genetics , Metabolism , TransfectionABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility of transferring fusion gene of dihydrofolate reductase (DHFR) gene and cytidine deaminase (CD) gene into mouse bone marrow cells in order to observe the drug resistance of high dose methotrexate (MTX) and cytosine arabinoside (Ara-C) in the bone marrow cells and to improve the tolerance of myelosuppression following combination chemotherapy.</p><p><b>METHODS</b>Human double-mutant dihydrofolate reductase-cytidine deaminase fusion gene was transferred into two mice bone marrow cells by retroviral vector. Resistant colony-forming unit granulocyte-macrophage (CFU-GM) assays were performed in mouse bone marrow cells by retroviral infection and after treatment by drugs (Ara-C, MTX, and Ara-C + MTX). DNA was extracted from mouse bone marrow cells. The expression of drug resistant genes in mouse bone marrow cells after transferring by retroviral vector was checked by polymerase chain reaction (PCR).</p><p><b>RESULTS</b>Bone marrow cells after coculture with the retroviral producer cells transduced with the genes (SFG-F/S-CD) showed the drug resistance colonies yield (Colony formation after exposure to Ara-C, MTX and Ara-C + MTX were 56%, 22% and 14%, respectively) and the increase in drug resistant to both MTX and Ara-C (P < 0.005). Expression of DHFR and CD gene in extracted DNA of transfected mice were demonstrated by PCR.</p><p><b>CONCLUSIONS</b>Double drug resistant gene can not only integrate and co-express in mice bone marrow cells but also increase the drug resistance to MTX and Ara-C.</p>